Mitigating Age-Related Ovarian Dysfunction with the Anti-Inflammatory Agent MIT-001.
Min-Hee KangYu Jin KimMin Jeong ChoJuYi JangYun Dong KooSoon Ha KimJae-Ho LeePublished in: International journal of molecular sciences (2023)
Ovarian aging is a major obstacle in assisted reproductive medicine because it leads to ovarian dysfunction in women of advanced age. Currently, there are no effective treatments to cure age-related ovarian dysfunction. In this study, we investigated the effect of MIT-001 on the function of aged ovaries. Young and old mice were utilized in this study. MIT-001 was intraperitoneally administered, and the number of follicles and oocytes was analyzed. Each group was then retrieved for RNA and protein isolation. Total RNA was subjected to mRNA next-generation sequencing. Protein extracts from ovarian lysates were used to evaluate various cytokine levels in the ovaries. MIT-001 enhanced follicles and the number of oocytes were compared with non-treated old mice. MIT-001 downregulated immune response-related transcripts and cytokines in the ovaries of old mice. MIT-001 modulates the immune complex responsible for generating inflammatory signals and has the potential to restore the function of old ovaries and improve female fertility.
Keyphrases
- oxidative stress
- immune response
- high fat diet induced
- anti inflammatory
- binding protein
- gene expression
- polycystic ovary syndrome
- pregnant women
- amino acid
- metabolic syndrome
- young adults
- mass spectrometry
- dna methylation
- high resolution
- copy number
- single molecule
- newly diagnosed
- circulating tumor cells
- human health