New Antifungal Metabolites from the Mariana Trench Sediment-Associated Actinomycete Streptomyces sp. SY1965.
Wenwen YiLe QinXiao-Yuan LianZhizhen ZhangPublished in: Marine drugs (2020)
New streptothiazolidine A (1), streptodiketopiperazines A (2) and B (3), and (S)-1-(3-ethylphenyl)-1,2-ethanediol (4), together with eight known compounds (5-12), were isolated from the Mariana Trench sediment-associated actinomycete Streptomyces sp. SY1965. The racemic mixtures of (±)-streptodiketopiperazine (2 and 3) and (±)-1-(3-ethylphenyl)-1,2-ethanediol (4 and 5) were separated on a chiral high-performance liquid chromatography (HPLC) column. Structures of the new compounds were elucidated by their high-resolution electrospray ionization mass spectroscopy (HRESIMS) data and extensive nuclear magnetic resonance (NMR) spectroscopic analyses. Streptothiazolidine A is a novel salicylamide analogue with a unique thiazolidine-contained side chain and its absolute configuration was established by a combination of nuclear Overhauser effect spectroscopy (NOESY) experiment, electronic circular dichroism (ECD) and 13C NMR calculations. New streptothiazolidine A (1) and streptodiketopiperazines A (2) and B (3) showed antifungal activity against Candida albicans with MIC values of 47, 42, and 42 g/mL, respectively.
Keyphrases
- high resolution
- candida albicans
- high performance liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- solid phase extraction
- magnetic resonance
- liquid chromatography
- simultaneous determination
- heavy metals
- ms ms
- biofilm formation
- capillary electrophoresis
- ionic liquid
- polycyclic aromatic hydrocarbons
- solid state
- molecular docking
- electronic health record
- molecular dynamics
- density functional theory
- molecular dynamics simulations
- organic matter
- single molecule
- contrast enhanced
- machine learning
- high speed
- big data
- risk assessment
- escherichia coli
- staphylococcus aureus
- cystic fibrosis
- computed tomography