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Pico-HPLC system integrating an equal inner diameter femtopipette into a 900 nm I.D. porous layer open tubular column.

Ruonan LiYunlong ShaoYanmin YuXiayan WangGuangsheng Guo
Published in: Chemical communications (Cambridge, England) (2018)
A picoflow high performance liquid chromatography (pico-HPLC) system was developed, which could directly pipette femtoliter samples using a separation column tip driven by an electroosmotic pump. Amino acid enantiomers were separated in the 900 nm I.D. porous layer open tubular column at a flow rate of 13.50 pL min-1.
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