Simulation-assisted DNA Nanodevice Serve as a General Optical Platform for Multiplexed Analysis of Micrornas.

Small frame nucleic acids (FNAs) serve as excellent carrier materials for various functional nucleic acid molecules, showcasing extensive potential applications in biomedicine development. The carrier module and function module combination is crucial for probe design, where an improper combination could significantly impede the functionality of sensing platforms. This study explores the effect of various combinations on the sensing performance of nanodevices through simulations and experimental approaches. We observed variances in response velocities, sensitivities and cell uptake efficiencies across different structures. Factors such as the number of functional molecules loaded, loading positions, and inter-modular distances affect the rigidity and stability of the nanostructure. Our findings reveal that the structures with full loads and moderate distances between modules has the lowest potential energy. Based on these insights, we developed a multi-signal detection platform that offers optimal sensitivity and response speed. This research offers valuable insights for designing FNAs-based probes and presents a streamlined method for the conceptualization and optimization of DNA nanodevices. This article is protected by copyright. All rights reserved.