Fast hybrid optomechanical scanning photoacoustic remote sensing microscopy for virtual histology.
Brendon S RestallBrendyn D CikalukMatthew T MartellNathaniel J M HavenRohan MittalSveta SilvermanLashan PeirisJean DeschenesBenjamin A AdamAdam KinnairdRoger J ZempPublished in: Biomedical optics express (2021)
A rapid scanning microscopy method for hematoxylin and eosin (H&E) like images is sought after for interoperative diagnosis of solid tumor margins. The rapid observation and diagnosis of histological samples can greatly lower surgical risk and improve patient outcomes from solid tumor resection surgeries. Photoacoustic remote sensing (PARS) has recently been demonstrated to provide images of virtual H&E stains with excellent concordance with true H&E staining of formalin-fixed, paraffin embedded tissues. By using PARS with constant velocity and 1D galvanometer mirror scanning we acquire large virtual H&E images (10mm x 5mm) of prostate tissue in less than 3.5 minutes without staining, and over two orders of magnitude faster data acquisition than the current PARS imaging speed.
Keyphrases
- high resolution
- optical coherence tomography
- deep learning
- convolutional neural network
- electron microscopy
- prostate cancer
- single molecule
- high speed
- mass spectrometry
- fluorescence imaging
- loop mediated isothermal amplification
- gene expression
- case report
- electronic health record
- high throughput
- big data
- blood flow
- label free
- machine learning