DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method.
Alexandra PoturnayováMonika BuríkováJozef BízikTibor HianikPublished in: Chemphyschem : a European journal of chemical physics and physical chemistry (2019)
By using the thickness shear mode acoustics method (TSM) and single-molecule force spectroscopy (SMFS) we studied the interactions between DNA aptamers (sgc8c) specific to the protein tyrosine kinase 7 (PTK7), which is localized in the membranes of leukemia lymphoblastics (MOLT-4), and lymphocyte (Jurkat) cell lines, as well with PTK7-negative U266 myeloid leukemia cells. The TSM method allowed the development of a highly sensitive, label-free biosensor for the detection leukemia cells with a limit of detection of (195±20) cells/mL. SMFS approved the high selectivity of the sgc8c aptamers to the PTK7 receptors at the cell surface and allowed determining the binding probability of the aptamers to the PTK7 receptors at different cell lines.
Keyphrases
- single molecule
- label free
- induced apoptosis
- cell cycle arrest
- acute myeloid leukemia
- tyrosine kinase
- bone marrow
- endoplasmic reticulum stress
- gold nanoparticles
- oxidative stress
- high resolution
- living cells
- cell death
- loop mediated isothermal amplification
- epidermal growth factor receptor
- dendritic cells
- binding protein