The budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are available. Here, we present a CAGE (cap analysis of gene expression) dataset for a-cells and α-cells grown in defined and rich media. Cell type-specific genes were differentially expressed as expected, ensuring the reliability of the data. Some of the differentially expressed TSSs were medium-specific or detected due to unrecognized chromosome rearrangement. By comparing the CAGE data with a high-resolution nucleosome map, major TSSs were primarily found in +1 nucleosomes, with a peak approximately 30 bp from the promoter-proximal end of the nucleosome. The dataset is available at DDBJ/GEA.
Keyphrases
- genome wide
- dna methylation
- saccharomyces cerevisiae
- gene expression
- high resolution
- transcription factor
- induced apoptosis
- copy number
- electronic health record
- single cell
- cell cycle arrest
- big data
- rna seq
- dna damage
- cell therapy
- mass spectrometry
- healthcare
- endoplasmic reticulum stress
- cell death
- single molecule
- data analysis
- machine learning
- social media
- tandem mass spectrometry
- high throughput sequencing