Directed Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin.

Emil HamnevikDirk MaurerThilak Reddy EnugalaThao ChuRobin LöfgrenDoreen DobritzschMikael Widersten

Published in: Biochemistry (2018)

Laboratory evolution of alcohol dehydrogenase produced enzyme variants with improved turnover numbers with a vicinal 1,2-diol and its corresponding hydroxyketone. Crystal structure and transient kinetics analysis aids in rationalizing the new functions of these variants.

Keyphrases

crystal structure
copy number
alcohol consumption
bone mineral density
antiretroviral therapy
cerebral ischemia
gene expression
dna methylation
data analysis