Near-Infrared Optical Imaging of Necrotic Cells by Photostable Lanthanide-Based Metallacrowns.
Ivana MartinićSvetlana V EliseevaTu N NguyenVincent L PecoraroStéphane PetoudPublished in: Journal of the American Chemical Society (2017)
Sensitive detection of cell necrosis is crucial for the determination of cell viability. Because of its high resolution at the cellular level and sensitivity, optical imaging is highly attractive for identifying cell necrosis. However, challenges associated with this technique remain present such as the rapid photobleaching of several types of organic fluorophores and/or the interference generated by biological autofluorescence. Herein, we synthesized novel biologically compatible Zn2+/Ln3+ metallacrowns (MCs) that possess attractive near-infrared (NIR) emission and are highly photostable. In addition, these MCs have the ability to label differentially necrotic HeLa cells from living cells. This work is also the first demonstration of (i) the use of the NIR emission arising from a single lanthanide(III) cation for optical biological imaging of cells under single photon excitation, (ii) the first example of a lanthanide(III)-based NIR-emitting probe that can be targeted to a specific type of cell.
Keyphrases
- high resolution
- fluorescent probe
- living cells
- sensitive detection
- single molecule
- single cell
- induced apoptosis
- cell cycle arrest
- quantum dots
- cell therapy
- energy transfer
- photodynamic therapy
- high speed
- fluorescence imaging
- mass spectrometry
- drug release
- heavy metals
- cell death
- stem cells
- ionic liquid
- loop mediated isothermal amplification
- bone marrow
- signaling pathway
- water soluble
- solid state