Specific detection of memory T-cells in COVID-19 patients using standardized whole-blood Interferon gammarelease assay.

William MoutonChristelle CompagnonKahina SakerSoizic DanielSophia DjebaliXavier LacouxBruno PozzettoGuy OriolDaphné LaubretonMargaux PrieuxJean-Baptiste FassierNicolas GuibertAmélie Massardier-PilonchéryDulce AlfaiateFranck BerthierThierry WalzerJacqueline MarvelKaren Brengel-PesceSophie Trouiller-Assant

Published in: European journal of immunology (2021)

Antigen-specific T-cells are essential for protective immunity against SARS-CoV-2. We set up a semi-automated whole-blood Interferon-gamma release assay (WB IGRA) to monitor the T-cell response after stimulation with SARS-CoV-2 peptide pools. We report that the WB IGRA is complementary to serological assays to assess SARS-CoV-2 immunity.

Keyphrases

sars cov
high throughput
respiratory syndrome coronavirus
dendritic cells
single cell
working memory
label free
real time pcr