Multi-Approach Characterization of Novel Pyrene-Degrading Mycolicibacterium austroafricanum Isolates Lacking nid Genes.
Natalia Maria SilvaCamila Lopes RomagnoliCaio Rafael do Nascimento SantiagoJoão Paulo Amorim de LacerdaSylvia Cardoso LeãoLuciano Antonio DigiampietriCristina Viana-NieroPublished in: Microorganisms (2023)
Polycyclic aromatic hydrocarbons (PAHs) are chemical compounds that are widespread in the environment, arising from the incomplete combustion of organic material, as well as from human activities involving petrol exploitation, petrochemical industrial waste, gas stations, and environmental disasters. PAHs of high molecular weight, such as pyrene, have carcinogenic and mutagenic effects and are considered pollutants. The microbial degradation of PAHs occurs through the action of multiple dioxygenase genes ( nid ), which are localized in genomic island denominate region A, and cytochrome P450 monooxygenases genes ( cyp ) dispersed in the bacterial genome. This study evaluated pyrene degradation by five isolates of Mycolicibacterium austroafricanum using 2,6-dichlorophenol indophenol (DCPIP assay), gas chromatography/mass spectrometry (CG/MS), and genomic analyses. Two isolates (MYC038 and MYC040) exhibited pyrene degradation indexes of 96% and 88%, respectively, over a seven-day incubation period. Interestingly, the genomic analyses showed that the isolates do not have nid genes, which are involved in PAH biodegradation, despite their ability to degrade pyrene, suggesting that degradation may occur due to the presence of cyp 150 genes, or even genes that have not yet been described. To the best of our knowledge, this is the first report of isolates without nid genes demonstrating the ability to degrade pyrene.
Keyphrases
- polycyclic aromatic hydrocarbons
- genome wide
- heavy metals
- bioinformatics analysis
- copy number
- healthcare
- genetic diversity
- dna methylation
- transcription factor
- gas chromatography mass spectrometry
- risk assessment
- endothelial cells
- human health
- mass spectrometry
- ms ms
- high throughput
- gene expression
- high resolution
- particulate matter
- simultaneous determination
- municipal solid waste
- induced pluripotent stem cells
- liquid chromatography