Comparison of data-acquisition methods for the identification and quantification of histone post-translational modifications on a Q Exactive HF hybrid quadrupole Orbitrap mass spectrometer.
Joby ColeEleanor J HansonDavid C JamesDavid H DockrellMark J DickmanPublished in: Rapid communications in mass spectrometry : RCM (2019)
We observed that DIA which offers advantages in flexibility and identification of isobaric peptide proteoforms performs as well as DDA in the analysis of histone PTMs. We were able to identify 71 modified histone peptides for histone H3 and H4 and quantified 64 across each of the different acquisition methods.
Keyphrases
- dna methylation
- mass spectrometry
- high resolution
- tandem mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- bioinformatics analysis
- gas chromatography
- electronic health record
- high resolution mass spectrometry
- high performance liquid chromatography
- big data
- gene expression
- heart failure
- simultaneous determination
- machine learning
- deep learning
- acute heart failure