Transcriptome-wide profiling of N 6 -methyladenosine via a selective chemical labeling method.

Studies of chemical modifications on RNA have ushered in the field of epitranscriptomics. N 6 -Methyladenosine (m 6 A) is the most typical RNA modification and is indispensable for basic biological processes. This study presents a chemical pulldown method (m 6 A-ORL-Seq) for transcriptome-wide profiling of m 6 A. Moreover, chemical labeling results in a specific reverse transcription (RT) truncation signature. This study has identified four thousand high-confidence m 6 A sites at single-base resolution in the human transcriptome. Unlike previously reported methods based on m 6 A-antibody or m 6 A-sensitive enzymes, the antibody/enzyme-free chemical method provides a new perspective for single-base m 6 A detection at the transcriptome level.