Profiling of immune dysfunction in COVID-19 patients allows early prediction of disease progression.
André F RendeiroJoseph CasanoCharles Kyriakos VorkasHarjot SinghAyana MoralesRobert A DeSimoneGrant B EllsworthRosemary SoaveShashi N KapadiaKohta SaitoChristopher D BrownJingMei HsuChristopher KyriakidesSteven ChiuLuca Vincenzo CappelliMaria Teresa CacciapuotiWayne TamLorenzo GalluzziPaul D SimonsonOlivier ElementoMirella SalvatoreGiorgio Ga InghiramiPublished in: Life science alliance (2020)
With a rising incidence of COVID-19-associated morbidity and mortality worldwide, it is critical to elucidate the innate and adaptive immune responses that drive disease severity. We performed longitudinal immune profiling of peripheral blood mononuclear cells from 45 patients and healthy donors. We observed a dynamic immune landscape of innate and adaptive immune cells in disease progression and absolute changes of lymphocyte and myeloid cells in severe versus mild cases or healthy controls. Intubation and death were coupled with selected natural killer cell KIR receptor usage and IgM+ B cells and associated with profound CD4 and CD8 T-cell exhaustion. Pseudo-temporal reconstruction of the hierarchy of disease progression revealed dynamic time changes in the global population recapitulating individual patients and the development of an eight-marker classifier of disease severity. Estimating the effect of clinical progression on the immune response and early assessment of disease progression risks may allow implementation of tailored therapies.
Keyphrases
- immune response
- single cell
- end stage renal disease
- newly diagnosed
- ejection fraction
- sars cov
- dendritic cells
- chronic kidney disease
- peritoneal dialysis
- healthcare
- induced apoptosis
- primary care
- oxidative stress
- toll like receptor
- risk factors
- cell death
- bone marrow
- signaling pathway
- climate change
- risk assessment
- cell proliferation
- inflammatory response
- cell cycle arrest
- binding protein
- cell therapy
- patient reported
- pi k akt