Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil.
Marcel Kruchelski TscháAndreia Akemi SuzukawaTiago GräfLaercio Dante Stein PianciniAllan Martins da SilvaHelisson FaoroIrina Nastassja RiedigerLia Carolina MedeirosPryscilla Fanini WowkCamila ZanlucaClaudia N Duarte Dos SantosPublished in: Emerging microbes & infections (2019)
In early 2017, an outbreak caused by an unknown and supposedly viral agent in the Marilena region of southern Brazil was investigated. Since the etiological agent causing the outbreak was not identified from human samples, mosquitoes from this region were collected. Three out of 121 mosquito pools collected from the region tested positive for alphavirus in molecular tests. Next generation sequencing results revealed the presence of a novel alphavirus, tentatively named here as Caainguá virus (CAAV). DNA barcoding analyses indicated that different species of Culex are hosts for CAAV. This new virus was basal to the New World encephalitic alphaviruses in a comprehensive and robust phylogenetic approach using complete genomes. Viral particles were observed in the cytosol and inside of intracellular compartments of cells in mosquito-derived cell cultures. Despite being noninfectious in vertebrate derived cell cultures, primary culturing of CAAV in human mononuclear cells suggests monocytes and lymphocytes as CAAV targets. However, the epidemiological link of CAAV on the human outbreak should be further explored.
Keyphrases
- endothelial cells
- aedes aegypti
- single cell
- induced apoptosis
- induced pluripotent stem cells
- sars cov
- cell therapy
- peripheral blood
- cell cycle arrest
- dengue virus
- circulating tumor
- gene expression
- oxidative stress
- zika virus
- dna methylation
- stem cells
- cell free
- signaling pathway
- cell death
- mesenchymal stem cells
- cell proliferation
- dendritic cells