Mechano-cytoskeleton remodeling mechanism and molecular docking studies on nanosurface technology - Titania nanotube arrays.

In biomedical implant technology, nanosurface such as titania nanotube arrays (TNA) could provide better cellular adaptation especially for long-term tissue acceptance response. Mechanotransduction activities of TNA nanosurface could involve the cytoskeleton remodelling mechanism. However, there is no clear insight into TNA mechano-cytoskeleton remodelling activities, especially computational approaches. Epithelial cells have played critical interface between biomedical implant surface and tissue exceptance particularly for long term interaction. Therefore, this study investigates genomic responses that are responsible for Cell-TNA mechano-stimulus using epithelial cells model. Findings suggested that Cell-TNA interaction may improve structural and extracellular matrix  (ECM) support on the cells as an adaptive response towards the nanosurface topography. More specifically, the surface topography of the TNA might improve the cell polarity and adhesion properties via the interaction of the plasma membrane and intracellular matrix responses. TNA nanosurface might engross the cytoskeleton remodelling activities for multi-directional cell movement and cellular protrusions on TNA nanosurface. These observations are supported by the molecular docking profiles that determine proteins' in silico binding mechanism on TNA. This active cell-surface revamping would allow cells to adapt develop a protective barrier towards TNA nanosurface, thus enhancing biocompatibility properties distinctly for long term interaction. The findings from this study will be beneficial towards nano-molecular knowledge of designing functional nano surface technology for advanced medical implant applications. This article is protected by copyright. All rights reserved.