Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.
Maike JoeresPavlo MaksimovDirk HöperSten CalvelageRafael Calero BernalMercedes Fernández-EscobarB KoudelaR BlagaM Globokar VrhovecKaya C StollbergNadja Seyhan BierSmaro SotirakiJacek SrokaW PiotrowskaPetr KodymWalter BassoFranz Josef ConrathsAurélien MercierLokman GalalMarie Laure DardéA BaleaF SpanoChristoph SchulzeMartin PetersN ScudaA LundénRebecca K DavidsonR TerlandHelga WaapE de BruinPaolo VattaSimone Mario CacciòLuis-Miguel Ortega-MoraJokelainen PikkaGereon ScharesPublished in: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology (2023)
The new method can distinguish different T. gondii genotypes and detect intra-genotype variability among European type II T. gondii strains. Furthermore, with WGS data additional target regions can be added to the method to potentially increase typing resolution.