Cesium could be used as a proxy for potassium in mycorrhizal <i>Medicago truncatula</i>.

Arbuscular mycorrhizal (AM) fungi interact with the roots of most land plants and help them to acquire various mineral resources from the soil, including potassium (K⁺). However, tracking K⁺ movement in AM symbiosis remains challenging. Recently, we reported that rubidium can be used as a proxy for K⁺ in mycorrhizal <i>Medicago truncatula</i>. In the present work, we investigated the possibility of using cesium (Cs⁺) as another proxy for K⁺ in AM symbiosis. Plants were placed in growing systems that include a separate compartment only accessible to the AM fungus <i>Rhizophagus irregularis</i> isolate 09 and in which various amounts of cesium chloride (0 mM, 0.5 mM, 1.5 mM, or 3.75 mM) were supplied. Plants were watered with sufficient K⁺ or K⁺-free nutrient solutions, and shoot and root biomass, fungal colonization, and K⁺ and Cs⁺ concentrations were recorded seven weeks after inoculation. Our results indicate that Cs⁺ accumulated in plant tissues only when K⁺ was present in the nutrient solution and when the highest concentration of Cs⁺ was used in the fungal compartment. Consequently, we conclude that Cs⁺ could be used as a proxy for K⁺ in AM symbiosis, but with serious limitations.