New Micropeptins with Anti-Neuroinflammatory Activity Isolated from a Cyanobacterial Bloom.
Riley D KirkHaiyin HePaul G WahomeShiBiao WuGuy T CarterMatthew J BertinPublished in: ACS omega (2021)
Metabolite mining of environmentally collected aquatic and marine microbiomes offers a platform for the discovery of new therapeutic lead molecules. Combining a prefractionated chromatography library with liquid chromatography tandem mass spectrometry (LC-MS/MS)-based molecular networking and biological assays, we isolated and characterized two new micropeptins (1 and 2) along with the previously characterized micropeptin 996. These metabolites showed potency in anti-neuroinflammatory assays using BV-2 mouse microglial cells, showing a 50% reduction in inflammation in a range from 1 to 10 μM. These results show promise for cyanobacterial peptides in the therapeutic realm apart from their impact on environmental health and provide another example of the utility of large prefractionated natural product libraries for therapeutic hit and lead identification.
Keyphrases
- liquid chromatography tandem mass spectrometry
- high throughput
- ms ms
- simultaneous determination
- induced apoptosis
- public health
- healthcare
- oxidative stress
- lps induced
- lipopolysaccharide induced
- mass spectrometry
- small molecule
- mental health
- high performance liquid chromatography
- solid phase extraction
- machine learning
- spinal cord injury
- cell proliferation
- high resolution
- neuropathic pain
- artificial intelligence
- amino acid
- bioinformatics analysis
- life cycle