DNA labelling in live cells via visible light-induced [2+2] photocycloaddition.

Rita T MichenfelderLaura Delafresnaye Vinh Xuan Truong Shivshankar R Mane Hans-Achim Wagenknecht

Published in: Chemical communications (Cambridge, England) (2023)

We introduce a visible light-driven ( λ max = 451 nm) photo-chemical strategy for labelling of DNA in living HeLa cells via the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.

Keyphrases

visible light
circulating tumor
cell free
induced apoptosis
cell cycle arrest
single molecule
nucleic acid
cell death
endoplasmic reticulum stress
circulating tumor cells
oxidative stress
quantum dots
high glucose
diabetic rats
light emitting