A new mouse SNP genotyping assay for speed congenics: combining flexibility, affordability, and power.

Kimberly R AndrewsSamuel S HunterBrandi K TorrevillasNora CéspedesSarah M GarrisonJessica StricklandDelaney WagersGretchen HanstenDaniel D NewMatthew W FagnanShirley Luckhart

Published in: BMC genomics (2021)

The SNP genotyping assay and bioinformatic pipeline developed here present a valuable tool for increasing the power and decreasing the cost of many studies that depend on speed congenics. The assay is highly flexible and can be used for combinations of strains that are commonly used for speed congenics. The assay could also be used for other techniques including QTL mapping, standard F2 crosses, ancestry analysis, and forensics.

Keyphrases

high throughput
genome wide
high density
genetic diversity
escherichia coli
single cell
high resolution
gene expression
data analysis
genome wide association study