Efficient Discrimination of Single Nucleotide Polymorphisms (SNPs) Using Oligonucleotides Modified with C5-Pyrene-Functionalized DNA and Flanking Locked Nucleic Acid (LNA) Monomers.
Mamta KauraPatrick J HrdlickaPublished in: Chemistry, an Asian journal (2016)
Oligodeoxyribonucleotides modified with 5-[3-(1-pyrenecarboxamido)propynyl]-2'-deoxyuridine monomer X and proximal LNA monomers display higher affinity for complementary DNA, more pronounced increases in fluorescence emission upon DNA binding, and improved discrimination of SNPs at non-stringent conditions, relative to the corresponding LNA-free probes across a range of sequence contexts. The results reported herein suggest that the introduction of LNA monomers influences the position of nearby fluorophores via indirect conformational restriction, a characteristic that can be utilized to develop optimized fluorophore-labeled probes for SNP-discrimination studies.
Keyphrases
- nucleic acid
- single molecule
- dna binding
- genome wide
- transcription factor
- molecularly imprinted
- molecular dynamics simulations
- living cells
- molecular dynamics
- circulating tumor
- quantum dots
- gene expression
- genome wide association
- computed tomography
- cell free
- fluorescence imaging
- fluorescent probe
- energy transfer
- genetic diversity
- amino acid
- circulating tumor cells