MoS 2 /PDA@Cu composite as a peroxidase-mimicking enzyme with high-effect antibacterial and anticancer activity.

Since nanozymes were proposed, their applications have become more and more extensive. As a research hotspot in recent years, MoS 2 also shows many enzyme-like properties. However, as a novel peroxidase, MoS 2 has the disadvantage of a low maximum reaction rate. In this study, the MoS 2 /PDA@Cu nanozyme was synthesized by a wet chemical method. The modification of PDA on the surface of MoS 2 achieved the uniform growth of small-sized Cu Nps. The obtained MoS 2 /PDA@Cu nanozyme displayed excellent peroxidase-like activity and antibacterial properties. The minimum inhibitory concentration (MIC) of the MoS 2 /PDA@Cu nanozyme against S. aureus reached 25 μg mL -1 . Furthermore, it showed a more pronounced inhibitory effect on bacterial growth with the addition of H 2 O 2 . The maximum reaction rate ( V max ) of the MoS 2 /PDA@Cu nanozyme is 29.33 × 10 -8 M s -1 , which is significantly higher as compared to that of HRP. It also exhibited excellent biocompatibility, hemocompatibility and potential anticancer properties. When the concentration of the nanozyme was 160 μg mL -1 , the viabilities of 4T1 cells and Hep G2 cells were 45.07% and 32.35%, respectively. This work indicates that surface regulation and electronic transmission control are good strategies for improving peroxidase-like activity.