Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis.
Jouha MinMaria NothingBen CobleHui ZhengJongmin ParkHyungsoon ImGeorg F WeberCesar M CastroFilip K SwirskiRalph WeisslederHakho LeePublished in: ACS nano (2018)
Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages (i) the pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis and (ii) a hybrid magneto-electrochemical sensor for IL-3 detection. The developed platform produces test results within 1 h from native blood samples and detects IL-3 at a sensitivity of <10 pg/mL; this performance is >5-times faster and >10-times more sensitive than conventional enzyme-linked immunoadsorbent assays, the current gold standard. Using clinical samples, we show that elevated plasma IL-3 levels are associated with high organ failure rate and thus greater risk of mortality, confirming the potential of IL-3 as a sepsis diagnostic biomarker. With further system development ( e. g., full automation, data security measures) and rigorous validation studies, the compact and fast IBS could be a practical clinical tool for timely diagnosis and proactive treatment of sepsis.
Keyphrases
- septic shock
- acute kidney injury
- intensive care unit
- loop mediated isothermal amplification
- label free
- gold nanoparticles
- high throughput
- type diabetes
- sensitive detection
- quantum dots
- coronary artery disease
- machine learning
- high resolution
- climate change
- deep learning
- cardiovascular events
- electronic health record
- combination therapy
- real time pcr
- low cost
- single cell
- clinical evaluation