Red light-triggered nucleic acid-templated reaction based on cyclic oligonucleotide substrates.
Oleksii ZozuliaTobias BachmannNina S Deussner-HelfmannFrank BeierleinMike HeilemannAndriy MokhirPublished in: Chemical communications (Cambridge, England) (2019)
A red light-triggered reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by specific nucleic acid templates and generates a bright fluorescent probe was developed. We confirmed that this reaction is compatible with fluorescence correlation spectroscopy (FCS) thereby allowing detection of nucleic acids down to 1 nM.