An activity-based probe targeting the streptococcal virulence factor C5a peptidase.
Sankarganesh KrishnamoorthyAndrea K SteigerWilliam C NelsonRobert G EgbertAaron T WrightPublished in: Chemical communications (Cambridge, England) (2022)
Development of profiling strategies to provide high resolution understanding of enzymes involved in bacterial infections remains an important need. These strategies help resolve enzyme mechanisms of actions and can guide therapeutic development. We have developed a selective new activity-based probe (ABP) targeting a highly conserved surface bound enzyme, C5a peptidase, present in several pathogenic Streptococci . We demonstrate our probe inhibits C5a peptidase activity and enables detection of C5a peptidase expressing pathogens in microbial mixtures. Our profiling strategy selectively labels the pathogen by phenotype and enables specific isolation of the live bacteria providing a route for further in-depth investigation. This study paves the way towards a rapid detection, isolation, and characterization pipeline for existing and emerging strains of most common pathogenic Streptococci .
Keyphrases
- loop mediated isothermal amplification
- high resolution
- escherichia coli
- living cells
- quantum dots
- cancer therapy
- staphylococcus aureus
- microbial community
- pseudomonas aeruginosa
- single cell
- transcription factor
- real time pcr
- optical coherence tomography
- ionic liquid
- drug delivery
- mass spectrometry
- multidrug resistant