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Intron lariat spliceosomes convert lariats to true circles: implications for intron transposition.

Manuel AresHaller IgelSol KatzmanJohn Paul Donohue
Published in: Genes & development (2024)
Rare, full-length circular intron RNAs distinct from lariats have been reported in several species, but their biogenesis is not understood. We envisioned and tested a hypothesis for their formation using Saccharomyces cerevisiae , documenting full-length and novel processed circular RNAs from multiple introns. Evidence implicates a previously undescribed catalytic activity of the intron lariat spliceosome (ILS) in which the 3'-OH of the lariat tail (with optional trimming and adenylation by the nuclear 3' processing machinery) attacks the branch, joining the intron 3' end to the 5' splice site in a 3'-5' linked circle. Human U2 and U12 spliceosomes produce analogous full-length and processed circles. Postsplicing catalytic activity of the spliceosome may promote intron transposition during eukaryotic genome evolution.
Keyphrases
  • left atrial appendage
  • saccharomyces cerevisiae
  • endothelial cells
  • dna methylation
  • gene expression
  • genome wide
  • dna repair
  • dna damage
  • oxidative stress
  • induced pluripotent stem cells
  • catheter ablation