High-affinity IgM+ memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.
Yasuyuki TashiroAkikazu MurakamiYasushi HaraTakeyuki ShimizuMasato KuboRyo GoitsukaHidehiro KishimotoTakachika AzumaPublished in: Scientific reports (2018)
IgM antibodies (Abs) are thought to play a major role in humoral immunity but only at the early stage of the primary immune response. However, two subsets of IgM+ memory B cells (MBCs), one with high affinity gained by means of multiple somatic hypermutation (SHM) and the other with low affinity and no SHMs, are generated through the germinal center (GC)-dependent and GC-independent (non-GC) pathway, respectively, after immunization with (4-hydroxy-3-nitrophenyl)acetyl (NP)-chicken γ-globulin. Surprisingly, an analysis of antibody-secreting cells reveals that a large amount of anti-NP IgM Ab with few SHMs is secreted during the recall response, indicating that only non-GC MBCs have terminal differentiation potential. Since secondary IgM Abs are capable of binding to dinitrophenyl ligands, they likely provide broad cross-reactivity in defense against microbial infection.
Keyphrases
- immune response
- induced apoptosis
- early stage
- cell cycle arrest
- gas chromatography
- working memory
- endoplasmic reticulum stress
- signaling pathway
- microbial community
- squamous cell carcinoma
- gene expression
- risk assessment
- radiation therapy
- cell death
- toll like receptor
- copy number
- dna methylation
- pi k akt
- lymph node
- cell proliferation
- neoadjuvant chemotherapy