HIV Promotes Atherosclerosis via Circulating Extracellular Vesicle MicroRNAs.

People living with HIV (PLHIV) are at a higher risk of having cerebrocardiovascular diseases (CVD) compared to HIV negative (HIV neg ) individuals. The mechanisms underlying this elevated risk remains elusive. We hypothesize that HIV infection results in modified microRNA (miR) content in plasma extracellular vesicles (EVs), which modulates the functionality of vascular repairing cells, i.e., endothelial colony-forming cells (ECFCs) in humans or lineage negative bone marrow cells (lin - BMCs) in mice, and vascular wall cells. PLHIV (N = 74) have increased atherosclerosis and fewer ECFCs than HIV neg individuals (N = 23). Plasma from PLHIV was fractionated into EVs (HIV posEVs ) and plasma depleted of EVs (HIV PL depEVs ). HIV posEVs , but not HIV PL depEVs or HIV negEVs (EVs from HIV neg individuals), increased atherosclerosis in apoE -/- mice, which was accompanied by elevated senescence and impaired functionality of arterial cells and lin - BMCs. Small RNA-seq identified EV-miRs overrepresented in HIV posEVs , including let-7b-5p. MSC (mesenchymal stromal cell)-derived tailored EVs (TEVs) loaded with the antagomir for let-7b-5p (miRZip-let-7b) counteracted, while TEVs loaded with let-7b-5p recapitulated the effects of HIV posEVs in vivo. Lin - BMCs overexpressing Hmga2 (a let-7b-5p target gene) lacking the 3'UTR and as such is resistant to miR-mediated regulation showed protection against HIV posEVs -induced changes in lin - BMCs in vitro. Our data provide a mechanism to explain, at least in part, the increased CVD risk seen in PLHIV.