An interdigitated aptasensor to detect interleukin-6 for diagnosing rheumatoid arthritis in serum.
Nan ChenHao YangQing LiLijun SongSubash C B GopinathDi WuPublished in: Biotechnology and applied biochemistry (2020)
Rheumatoid arthritis (RA) is an autoimmune disorder causing chronic inflammation in the small joints of the articular bone and destruction of articular cartilage. RA causes stiffness, pain, joint destruction, substantial comorbidity, and functional disability. Early-stage diagnosis of RA can help in the treatment of the disease and expand the patient life span. Interleukins are a group of inflammatory cytokines; in particular, an abundance of interleukin-6 (IL-6) was found in the synovial fluid and serum. In RA patients, the levels of IL-6 have been found to be correlated with the disease, and this work focused on detecting IL-6 by its aptamer with the help of a biotin-streptavidin strategy on an interdigitated electrode. A sensitivity of 1 fM (0.021 pg/mL) and a limit of detection of 10 fM (0.21 pg/mL) were found by a linear regression [y = 0.6413x - 0.6249; R² = 0.952] of the linear range from 1 fM to 100 pM. This method enhanced the immobilization of higher aptamer molecules for recognizing RA in serum-containing samples and is applicable to other diseases.
Keyphrases
- rheumatoid arthritis
- disease activity
- early stage
- ankylosing spondylitis
- label free
- interstitial lung disease
- end stage renal disease
- sensitive detection
- multiple sclerosis
- systemic lupus erythematosus
- gold nanoparticles
- chronic kidney disease
- oxidative stress
- chronic pain
- newly diagnosed
- peritoneal dialysis
- ejection fraction
- air pollution
- heavy metals
- case report
- loop mediated isothermal amplification
- particulate matter
- polycyclic aromatic hydrocarbons
- spinal cord injury
- risk assessment
- sentinel lymph node
- combination therapy
- neural network
- real time pcr
- energy transfer