Micropipet-Based Navigation in a Microvascular Model for Imaging Endothelial Cell Topography Using Scanning Ion Conductance Microscopy.
Noriko TairaYuji NashimotoKosuke InoHiroki IdaTakuto ImaizumiAkichika KumataniYasufumi TakahashiHitoshi ShikuPublished in: Analytical chemistry (2021)
Scanning ion conductance microscopy (SICM) has enabled cell surface topography at a high resolution with low invasiveness. However, SICM has not been applied to the observation of cell surfaces in hydrogels, which can serve as scaffolds for three-dimensional cell culture. In this study, we applied SICM for imaging a cell surface in a microvascular lumen reconstructed in a hydrogel. To achieve this goal, we developed a micropipet navigation technique using ionic current to detect the position of a microvascular lumen. Combining this navigation technique with SICM, endothelial cells in a microvascular model and blebs were visualized successfully at the single-cell level. To the best of our knowledge, this is the first report on visualizing cell surfaces in hydrogels using a SICM. This technique will be useful for furthering our understanding of the mechanism of intravascular diseases.
Keyphrases
- high resolution
- cell surface
- single cell
- endothelial cells
- tissue engineering
- drug delivery
- rna seq
- hyaluronic acid
- mass spectrometry
- high speed
- high throughput
- cell therapy
- healthcare
- single molecule
- biofilm formation
- coronary artery
- ultrasound guided
- optical coherence tomography
- mesenchymal stem cells
- staphylococcus aureus
- stem cells
- electron microscopy
- vascular endothelial growth factor
- bone marrow