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Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei.

Karen BillingtonClare HallidayRoss MaddenPhilip DyerAmy Rachel BarkerFlávia Fernandes Moreira-LeiteMark CarringtonSue VaughanChristiane Hertz-FowlerSamuel DeanJack Daniel SunterRichard John WheelerKeith Gull
Published in: Nature microbiology (2023)
Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and plant unicellular parasites. Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. We provide clues to function and define lineage-specific organelle adaptations for parasitism, mapping the ultraconserved cellular architecture of eukaryotes, including the first comprehensive 'cartographic' analysis of the eukaryotic flagellum, which is vital for morphogenesis and pathology. To demonstrate the power of this resource, we identify novel organelle subdomains and changes in molecular composition through the cell cycle. TrypTag is a transformative resource, important for hypothesis generation for both eukaryotic evolutionary molecular cell biology and fundamental parasite cell biology.
Keyphrases
  • cell cycle
  • genome wide
  • single cell
  • life cycle
  • cell therapy
  • single molecule
  • high resolution
  • endothelial cells
  • cell proliferation
  • plasmodium falciparum
  • small molecule
  • mesenchymal stem cells
  • quantum dots