Clinical Investigation of Leukocyte DNA Damage in COVID-19 Patients.
Hasan DoğanAslı KaraErdem ÇankayaEda BalkanMuhammet Ali GürbüzMurat KızılkayaMerve AykaçPublished in: Current issues in molecular biology (2023)
This prospective cross-sectional study aimed to evaluate leukocyte DNA damage in coronavirus disease (COVID-19) patients. In this study, 50 COVID-19-positive patients attending the Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included. DNA damage was detected in living cells through leukocyte isolation in 50 COVID-19-positive patients using the comet assay method. DNA tail/head (olive) moments were evaluated and compared. White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), neutrophils (NEU), lymphocytes (LYM), eosinophils (EO), monocytes (MONO), basophils (BASO), platelets (PLT), and the neutrophil/lymphocyte ratio (NLR) were analyzed. The RBC, lymphocyte, eosinophil, and monocyte means were significantly higher in the control group ( p < 0.05), whereas the HGB and neutrophile means were significantly higher in the study group ( p < 0.05). There were significant negative correlations between COVID-19 and RBC (r = -0.863), LYM (r = -0.542), EO (r = -0.686), and MONO (r = -0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r = 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582). Both the tail and olive moment mean differences were significantly higher in the study group, with higher ranges ( p < 0.05). COVID-19 infection caused statistically significant increases in both the tail and olive damage percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence and progression of COVID-19.
Keyphrases
- coronavirus disease
- dna damage
- sars cov
- end stage renal disease
- chronic kidney disease
- ejection fraction
- newly diagnosed
- oxidative stress
- red blood cell
- prognostic factors
- peripheral blood
- living cells
- primary care
- emergency department
- dendritic cells
- cell proliferation
- induced apoptosis
- high throughput
- patient reported
- nucleic acid
- acute care