Identification and functional analysis of a novel chorion protein essential for egg maturation in the brown planthopper.
Y-H LouP-L PanY-X YeC ChengH-J XuChuan-Xi ZhangPublished in: Insect molecular biology (2018)
In insect eggs, the chorion has the essential function of protecting the embryo from external agents during development while allowing gas exchange for respiration. In this study, we found a novel gene, Nilaparvata lugens chorion protein (NlChP), that is involved in chorion formation in the brown planthopper, Nilaparvata lugens. NlChP was highly expressed in the follicular cells of female adult brown planthoppers. Knockdown of NlChP resulted in oocyte malformation and the inability to perform oviposition, and electron microscopy showed that the malformed oocytes had thin and rough endochorion layers compared to the control group. Liquid chromatography with tandem mass spectrometry analysis of the eggshell components revealed four unique peptides that were matched to NlChP. Our results demonstrate that NlChP is a novel chorion protein essential for egg maturation in N. lugens, a hemipteran insect with telotrophic meroistic ovaries. NlChP may be a potential target in RNA interference-based insect pest management.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- aedes aegypti
- mass spectrometry
- amino acid
- high performance liquid chromatography
- simultaneous determination
- high resolution mass spectrometry
- gas chromatography
- induced apoptosis
- electron microscopy
- binding protein
- solid phase extraction
- high resolution
- risk assessment
- zika virus
- cell cycle arrest
- single cell
- transcription factor
- oxidative stress
- human health
- room temperature
- cell proliferation
- ms ms
- endoplasmic reticulum stress
- climate change
- gene expression