GdPO4-Based Nanoprobe for Bioimaging and Selective Recognition of Dipicolinic Acid and Cysteine by a Sensing Ensemble Approach.
Jun XuXiaoke ShenLei JiaZhijun GeDong ZhouYong YangTieliang MaYifeng LuoTaofeng ZhuPublished in: ACS biomaterials science & engineering (2019)
Multiple functions incorporated in one single-component nanoplatform pave the way for important biomedicine applications. Herein, a multifunctional terbium-doped gadolinium orthophosphate (GdPO4:Tb-EDTA) nanoplatform was prepared through a simple, ecofriendly, one-step hydrothermal method. Results showed that dipicolinic acid (DPA), the biomarker of bacterial spores, significantly increased the fluorescence intensity of this nanoplatform and conferred it with rapid response and excellent selectivity. Subsequently, the fluorescence of the ensemble GdPO4:Tb-EDTA-DPA can be remarkably quenched by Cu2+, which led to a rewritable nanosensor used in the detection of cysteine (Cys) with excellent sensitivity. In addition, GdPO4:Tb-EDTA can also be a potential T1-weighted magnetic resonance imaging (MRI) contrast agent, which indicated a satisfactory in vitro MRI with r1 relaxivity values of 13.9 mM-1 s-1 and in vivo MRI through intravenous administration on a rat model. Overall, the proposed assay may have great theoretical and practical significance for designing multifunctional biomaterials.
Keyphrases
- contrast enhanced
- magnetic resonance imaging
- cancer therapy
- magnetic resonance
- living cells
- photodynamic therapy
- mycobacterium tuberculosis
- diffusion weighted imaging
- drug delivery
- computed tomography
- fluorescent probe
- single molecule
- drug release
- quantum dots
- metal organic framework
- loop mediated isothermal amplification
- high intensity
- high dose
- convolutional neural network
- high throughput
- risk assessment
- low dose
- climate change
- neural network
- human health
- tissue engineering