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Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing.

Michael J ZillerKasper D HansenAlexander MeissnerMartin J Aryee
Published in: Nature methods (2014)
Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates.
Keyphrases
  • single cell
  • dna methylation
  • genome wide
  • gene expression
  • healthcare
  • big data
  • artificial intelligence