Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum.

Ya-Jun LiuShiyue LiuSheng DongRenmin LiYingang FengQiu Cui

Published in: Biotechnology for biofuels (2018)

Our results confirmed that the high activity and substrate specificity of Cel48S_CD from C. thermocellum were consistent with its importance in the cellulosome. The structure of the native Cel48S_CD protein revealed evidence of conformational changes during substrate binding. In addition, our study provided a reliable method for in situ purification of cellulosomal and other secretive proteins from C. thermocellum.

Keyphrases

amino acid
structural basis
molecular dynamics
molecular dynamics simulations
binding protein
single cell
protein protein
small molecule
molecularly imprinted
solid phase extraction
mass spectrometry
high resolution
recombinant human