Protective Effect of Baicalin Against TLR4-mediated UVA-induced Skin Inflammation.
Mohammad Asif SherwaniKevin YangAditi JaniReham A AbedAbdul Karim TaufiqueTolulope Gid DosunmuNabiha YusufPublished in: Photochemistry and photobiology (2018)
UVA irradiation is known to cause photoaging via production of reactive oxygen species (ROS) and activation of inflammatory processes. Previously, we have demonstrated that baicalin, a plant-derived flavonoid possessing both antioxidant and anti-inflammatory activity, protects mouse keratinocytes against damage from UVB irradiation. However, the role of baicalin in vivo has not been well studied, particularly in the setting of UVA irradiation. To explore the protective effects and mechanisms of baicalin treatment in mice after UVA irradiation, mice were exposed to acute and chronic doses of UVA irradiation with or without baicalin or vehicle. Skin samples were collected for histological staining, RNA isolation, flow cytometry and protein extraction. Our results demonstrate the protective effect of baicalin against UVA-induced oxidative damage and inflammation in mouse skin. These effects are likely mediated via the TLR4 pathway, which may serve as a target for photochemoprevention against skin inflammation.
Keyphrases
- oxidative stress
- diabetic rats
- flow cytometry
- wound healing
- reactive oxygen species
- soft tissue
- drug induced
- toll like receptor
- inflammatory response
- dna damage
- high glucose
- radiation induced
- immune response
- liver failure
- high fat diet induced
- nuclear factor
- adipose tissue
- small molecule
- skeletal muscle
- protein protein
- extracorporeal membrane oxygenation
- binding protein
- intensive care unit
- wild type