Loss of FBXO7 (PARK15) results in reduced proteasome activity and models a parkinsonism-like phenotype in mice.
Siv VingillDavid BrockeltCamille LancelinLars TatenhorstGuergana DontchevaChristian PreisingerNicola Schwedhelm-DomeyerSabitha JosephMiso MitkovskiSandra GoebbelsKlaus-Armin NaveJörg B SchulzTill MarquardtPaul LingorJudith StegmüllerPublished in: The EMBO journal (2016)
Mutations in the FBXO7 (PARK15) gene have been implicated in a juvenile form of parkinsonism termed parkinsonian pyramidal syndrome (PPS), characterized by Parkinsonian symptoms and pyramidal tract signs. FBXO7 (F-box protein only 7) is a subunit of the SCF (SKP1/cullin-1/F-box protein) E3 ubiquitin ligase complex, but its relevance and function in neurons remain to be elucidated. Here, we report that the E3 ligase FBXO7-SCF binds to and ubiquitinates the proteasomal subunit PSMA2. In addition, we show that FBXO7 is a proteasome-associated protein involved in proteasome assembly. In FBXO7 knockout mice, we find reduced proteasome activity and early-onset motor deficits together with premature death. In addition, we demonstrate that NEX (neuronal helix-loop-helix protein-1)-Cre-induced deletion of the FBXO7 gene in forebrain neurons or the loss of FBXO7 in tyrosine hydroxylase (TH)-positive neurons results in motor defects, reminiscent of the phenotype in PARK15 patients. Taken together, our study establishes a vital role for FBXO7 in neurons, which is required for proper motor control and accentuates the importance of FBXO7 in proteasome function.
Keyphrases
- early onset
- spinal cord
- transcription factor
- binding protein
- end stage renal disease
- late onset
- copy number
- chronic kidney disease
- gene expression
- parkinson disease
- drug induced
- pet ct
- computed tomography
- peritoneal dialysis
- prognostic factors
- adipose tissue
- dna methylation
- blood brain barrier
- diabetic rats
- endothelial cells
- depressive symptoms
- protein kinase
- positron emission tomography
- pet imaging