A Comparison of the Performance of Modular Standalone Do-It-Yourself Ion Mobility Spectrometry Systems.

As the spectrum of ion mobility spectrometry (IMS) applications expands and more experimental configurations are developed, identifying the correct platform for an experimental campaign becomes more challenging for researchers. Additionally, metrics that compare performance ( R p, for example) often have nuanced differences in definition between platforms that render direct comparisons difficult. Here we present a comparison of three do-it-yourself (DIY) drift tubes that are relatively low cost and easy to construct, where the performance of each is evaluated based on three different metrics: resolving power, the ideality of resolving powers, and accuracy/precision of K 0 values. The standard PCBIMS design developed by Reinecke and Clowers (Reinecke, T.; Clowers, B. H. HardwareX 2018, 4, e00030) provided the highest resolving power (>90) and the highest ideality of resolving power ratios (>90% at best) of the three systems. However, the flexible tube (FlexIMS) construction as described by Smith et al. (Smith, B. L. Anal. Chem. 2020, 92 (13), 9104-9112) exhibited the highest degree of precision of K 0 values (relative standard deviation of <0.42%). Depending on the application, the drift tube variants presented and evaluated here offer a low-cost alternative to commercial drift-tube systems with levels of performance that approach theoretical maxima.