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Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer.

Sebastian IsbanerNarain KaredlaIzabela KamińskaDaja RuhlandtMario RaabJohann BohlenAlexey I ChizhikIngo GregorPhilip TinnefeldJörg EnderleinRoman Tsukanov
Published in: Nano letters (2018)
Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule metal-induced energy transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm. smMIET relies only on fluorescence lifetime measurements and does not require additional complex optical setups.
Keyphrases
  • single molecule
  • energy transfer
  • atomic force microscopy
  • high resolution
  • quantum dots
  • living cells
  • high speed
  • high glucose
  • diabetic rats
  • drug induced
  • photodynamic therapy
  • endothelial cells