Sperm preparation by density gradient centrifugation, an extended horizontal swim up or both? Evaluation of sperm DNA fragmentation, sperm chromatin decondensation and embryo quality.

Malak JamilHasnae DebbarhAmal KabitMohamed EnnajiLoic KoumbaIsmail KaarouchMohamed ZarqaouiWassim Rhazi SenhajiEl Mehdi HissaneBrahim SaadaniPierre VanderzwalmenNourredine LouanjliRachida Cadi

Published in: Obstetrics & gynecology science (2023)

The use of combination of DGC and extended horizontal SU techniques significantly lowered the rates of DNA fragmentation and chromatin decondensation, and DGC alone of normozoospermic sperm might be sufficient for healthy embryo development.

Keyphrases

circulating tumor
gene expression
dna damage
transcription factor
cell free
single molecule
genome wide
pregnancy outcomes
pregnant women
nucleic acid
high resolution
circulating tumor cells
liquid chromatography
tandem mass spectrometry