Nanoscale photobiotinylation, pulldown and sequencing of region-specific DNA from intact cells.
Thomas C RobertsMax KushnerJack C CrowleyAbdullah OzerJuan WangJudhajeet RayJohn T LisWarren R ZipfelPublished in: bioRxiv : the preprint server for biology (2023)
Femto-seq is a novel nanoscale optical method that can be used to obtain DNA sequence information from targeted regions around a specific locus or other nuclear regions of interest. Two-photon excitation is used to photobiotinylate femtoliter volumes of chromatin within the nucleus, allowing for subsequent isolation and sequencing of DNA, and bioinformatic mapping of any nuclear region of interest in a select set of cells from a heterogenous population.
Keyphrases
- circulating tumor
- cell free
- single molecule
- single cell
- high resolution
- atomic force microscopy
- induced apoptosis
- genome wide
- gene expression
- nucleic acid
- rna seq
- transcription factor
- cell cycle arrest
- cancer therapy
- high speed
- circulating tumor cells
- healthcare
- health information
- mass spectrometry
- oxidative stress
- amino acid
- genome wide association study
- monte carlo