Immunogenicity of HLA-DR1 and HLA-A2 peptides derived from Leishmania major Gp63 in golden hamsters.
Larissa Pinheiro SilvaMauricio Oviedo PacielloWéllida Patricia Aviz TeixeiraAçucena Veleh RivasRaimundo Wagner Souza AgularAlex Sander Rodrigues CangussuLuiz Carlos Bertucci BarbosaReinaldo MarchettoRodolfo Cordeiro GiunchettiKelvinson Fernandes VianaPublished in: Parasite immunology (2020)
Through bioinformatics analyses, two Leishmania major Gp63 peptides were predicted and selected for vaccine formulations. Hamsters were divided into four groups, with each group receiving doses of three vaccine formulations containing HLA-DR1 or HLA-A2 peptides plus MontanideTM or both associated with the adjuvant. The animals received three vaccine doses and were evaluated for toxicity after each dose, in addition to being analysed for the production of antibodies and lymphoproliferation on day 211 after the last vaccine dose. Peptides predicted in association with oily adjuvant induced a humoral response and strong lymphoproliferation to Leishmania infantum antigen-specific stimulation.