Nandrolone and estradiol biomarkers identification in bovine urine applying a liquid chromatography high-resolution mass spectrometry metabolomics approach.
Saadia OuziaAnne-Lise RoyerMarzia PezzolatoAlessandro BenedettoElena BiasibettiYann GuittonBruno Le BizecElena BozettaGaud DervillyPublished in: Drug testing and analysis (2021)
With the aim of specifically investigating patterns associated with three steroid treatments (17β-nandrolone, 17β-estradiol, and 17β-nandrolone + 17β-estradiol) in bovine, an reversed phase liquid chromatography (RPLC)-electrospray ionization (ESI)(+/-)-high-resolution mass spectrometry (HRMS) study was conducted to characterize the urinary profiles of involved animals. Although specific fingerprints with strong differences could be highlighted between urinary metabolite profiles within urine samples collected on control and treated animals, it appeared further that significant discriminations could also be observed between steroid treatments, evidencing thus specific patterns and candidate biomarkers associated to each treatment. An MS-2 structural elucidation step enabled level-1 identification of two biomarkers mainly involved in energy pathways, in relation to skeletal muscle functioning. These results make it possible to envisage a global strategy for the detection of anabolic practices involving steroids, while at the same time providing clues as to the compounds used, which would facilitate the confirmation stage to follow.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- mass spectrometry
- ultra high performance liquid chromatography
- tandem mass spectrometry
- gas chromatography
- skeletal muscle
- simultaneous determination
- ms ms
- estrogen receptor
- healthcare
- solid phase extraction
- primary care
- high resolution
- bioinformatics analysis
- insulin resistance
- type diabetes
- multiple sclerosis
- adipose tissue
- smoking cessation
- quantum dots