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Single-cell characterization of a model of poly I:C-stimulated peripheral blood mononuclear cells in severe asthma.

Ailu ChenMaria P Diaz-SotoMiguel F SanmamedTaylor AdamsJonas C SchuppAmolika GuptaClemente BrittoMaor SaulerXiting YanQing LiuGustavo NinoCharles S Dela CruzGeoffrey L ChuppJose L Gomez
Published in: Respiratory research (2021)
Single-cell profiling of an in vitro model using PBMCs in patients with SA identified activation of pro-inflammatory pathways at baseline and strong response to Poly I:C, as well as quantitative changes in CD8 + effector cells. Thus, transcriptomic and cell quantitative changes are associated with immune cell heterogeneity in this model to evaluate interferon responses in severe asthma.
Keyphrases
  • single cell
  • rna seq
  • high throughput
  • induced apoptosis
  • high resolution
  • regulatory t cells
  • oxidative stress
  • signaling pathway
  • mass spectrometry
  • endoplasmic reticulum stress