Single-cell characterization of a model of poly I:C-stimulated peripheral blood mononuclear cells in severe asthma.
Ailu ChenMaria P Diaz-SotoMiguel F SanmamedTaylor AdamsJonas C SchuppAmolika GuptaClemente BrittoMaor SaulerXiting YanQing LiuGustavo NinoCharles S Dela CruzGeoffrey L ChuppJose L GomezPublished in: Respiratory research (2021)
Single-cell profiling of an in vitro model using PBMCs in patients with SA identified activation of pro-inflammatory pathways at baseline and strong response to Poly I:C, as well as quantitative changes in CD8 + effector cells. Thus, transcriptomic and cell quantitative changes are associated with immune cell heterogeneity in this model to evaluate interferon responses in severe asthma.