Single-cell characterization of a model of poly I:C-stimulated peripheral blood mononuclear cells in severe asthma.

Ailu ChenMaria P Diaz-SotoMiguel F SanmamedTaylor AdamsJonas C SchuppAmolika GuptaClemente BrittoMaor SaulerXiting YanQing LiuGustavo NinoCharles S Dela CruzGeoffrey L ChuppJose L Gomez

Published in: Respiratory research (2021)

Single-cell profiling of an in vitro model using PBMCs in patients with SA identified activation of pro-inflammatory pathways at baseline and strong response to Poly I:C, as well as quantitative changes in CD8 + effector cells. Thus, transcriptomic and cell quantitative changes are associated with immune cell heterogeneity in this model to evaluate interferon responses in severe asthma.

Keyphrases

single cell
rna seq
high throughput
induced apoptosis
high resolution
regulatory t cells
oxidative stress
signaling pathway
mass spectrometry
endoplasmic reticulum stress