Investigating eosin Y as a photocatalyst for the radical-dependent activity-based probing of deubiquitinating enzymes.
Neil C TaylorJoanna F McGouranPublished in: Organic & biomolecular chemistry (2021)
Eosin Y was assessed for its ability to induce a thiol-ene dependent protein-protein reaction in a metal-free, oxygen-tolerant, visible light mediated system. Protein-protein coupling efficiency under these mild conditions was comparable to previously reported UV-dependent conditions. The desired thiol-ene reaction was however limited within more complex biological systems.