Insights into the Oxidative Stress Response of Salmonella enterica serovar Enteritidis Revealed by the Next Generation Sequencing Approach.
Xiaoying LiuMisara OmarJuan E AbrahanteKakambi V NagarajaSinisa VidovicPublished in: Antioxidants (Basel, Switzerland) (2020)
As a facultative intracellular pathogen, Salmonella Enteritidis must develop an effective oxidative stress response to survive exposure to reactive oxygen species within the host. To study this defense mechanism, we carried out a series of oxidative stress assays in parallel with a comparative transcriptome analyses using a next generation sequencing approach. It was shown that the expression of 45% of the genome was significantly altered upon exposure to H2O2. Quantitatively the most significant (≥100 fold) gene expression alterations were observed among genes encoding the sulfur utilization factor of Fe-S cluster formation and iron homeostasis. Our data point out the multifaceted nature of the oxidative stress response. It includes not only numerous mechanisms of DNA and protein repair and redox homeostasis, but also the key genes associated with osmotic stress, multidrug efflux, stringent stress, decrease influx of small molecules, manganese and phosphate starvation stress responses. Importantly, this study revealed that oxidatively stressed S. Enteritidis cells simultaneously repressed key motility encoding genes and induced a wide range of adhesin- and salmonellae-essential virulence-encoding genes, that are critical for the biofilm formation and intracellular survival, respectively. This finding indicates a potential intrinsic link between oxidative stress and pathogenicity in non-typhoidal Salmonella that needs to be empirically evaluated.
Keyphrases
- oxidative stress
- biofilm formation
- induced apoptosis
- diabetic rats
- escherichia coli
- gene expression
- genome wide
- reactive oxygen species
- pseudomonas aeruginosa
- candida albicans
- staphylococcus aureus
- dna damage
- ischemia reperfusion injury
- circulating tumor
- copy number
- endoplasmic reticulum stress
- cystic fibrosis
- cell cycle arrest
- bioinformatics analysis
- high throughput
- cell proliferation
- multidrug resistant
- small molecule
- electronic health record
- big data
- heat stress
- climate change
- genome wide identification
- pi k akt
- deep learning
- data analysis
- electron transfer