Next-generation sequencing of cytologic preparations: An analysis of quality metrics.

David H HwangElizabeth P GarciaMatthew D DucarEdmund S CibasLynette M Sholl

Published in: Cancer cytopathology (2017)

The adequacy rate of cytology smears/LBPs for NGS is comparable to that of core BXs or CBs. Increased values for the mean insert size, mean target coverage, and percent usable bases, along with a lower duplication rate, suggest that smears/LBPs provide higher quality DNA than formalin-fixed material. Cytology smears/LBPs can serve as a valuable source of material for molecular testing. Cancer Cytopathol 2017;125:786-94. © 2017 American Cancer Society.

Keyphrases

papillary thyroid
fine needle aspiration
squamous cell
circulating tumor
high grade
single molecule
ultrasound guided
squamous cell carcinoma
quality improvement
childhood cancer
copy number
gene expression