Replication efficiencies of human cytomegalovirus-infected epithelial cells are dependent on source of virus production.
Rebekah L MokryChristopher E MontiSuzette Rosas-RogersMegan L SchumacherRanjan K DashScott S TerhunePublished in: bioRxiv : the preprint server for biology (2024)
Human cytomegalovirus (HCMV) is a prevalent betaherpesvirus, and infection can lead to a range of symptomatology from mononucleosis to sepsis in immunocompromised individuals. HCMV is also the leading viral cause of congenital birth defects. Lytic replication is supported by many cell types with different kinetics and efficiencies leading to a plethora of pathologies. The goal of these studies was to elucidate HCMV replication efficiencies for viruses produced on different cell types upon infection of epithelial cells by combining experimental approaches with data-driven computational modeling. HCMV was generated from a common genetic background of TB40-BAC4, propagated on fibroblasts (TB40 Fb ) or epithelial cells (TB40 Epi ), and used to infect epithelial cells. We quantified cell-associated viral genomes (vDNA), protein levels (pUL44, pp28), and cell-free titers over time for each virus at different multiplicities of infection. We combined experimental quantification with data-driven simulations and determined that parameters describing vDNA synthesis were similar between sources. We found that pUL44 accumulation was higher in TB40 Fb than TB40 Epi . In contrast, pp28 accumulation was higher in TB40 Epi which coincided with a significant increase in titer for TB40 Epi over TB40 Fb . These differences were most evident during live-cell imaging, which revealed syncytia-like formation during infection by TB40 Epi . Simulations of the late lytic replication cycle yielded a larger synthesis constant for pp28 in TB40 Epi along with increase in virus output despite similar rates of genome synthesis. By combining experimental and computational modeling approaches, our studies demonstrate that the cellular source of propagated virus impacts viral replication efficiency in target cell types.
Keyphrases
- mycobacterium tuberculosis
- single cell
- cell therapy
- endothelial cells
- cell free
- sars cov
- stem cells
- intensive care unit
- computed tomography
- high resolution
- gene expression
- epstein barr virus
- genome wide
- molecular dynamics
- acute kidney injury
- pregnant women
- small molecule
- monte carlo
- extracellular matrix
- diffuse large b cell lymphoma
- aqueous solution